Journal: Cellular Oncology

Article Title: Single-cell transcriptomic mapping of patient-derived primary liver cancer organoids reveals molecular subtypes and guides precision drug targeting

doi: 10.1007/s13402-026-01190-w

Figure Lengend Snippet: Modulating the cellular mitochondrial pathway is crucial for the treatment of CHC. ( A ) UMAP visualization depicting the spatial distribution of individual cell populations within CHC organoids. ( B ) Slingshot trajectory analysis illustrating the dynamic progression of CHC organoid cells. ( C ) Monocle-derived trajectory plots showcasing the developmental derivation of CHC organoids, inferred from Pseudotime and monocle clusters. ( D ) Gene trajectory plots of key genes, illustrating their expression patterns across developmental stages. ( E ) Pseudotime heatmap highlighting distinct clusters and their temporal dynamics. ( F - G ) GSEA enrichment analysis of CHC organoid enrichment genes. ( H - I ) Representative images of CHC organoids in the regorafenib group and control group observed by bright-field microscopy and H&E staining(scale bar, 50 μm). ( J - M ) Two CHC organoids were evaluated by JC-1 assay(scale bar, 50 μm). This was followed by quantifying the relative fluorescence intensity. ( N ) The mitochondrial structure of two CHC organoids was observed under electron microscope(scale bar, 1 μm). In all statistical plots, data are expressed as the mean ± SD, t-test (Fig. H and I) Two-way ANOVA (Fig. 7K and M) were used to determine statistical significance. (* p < 0.05, ** P < 0.001, *** P < 0.001, n = 3)

Article Snippet: Follow the instructions for the JC-1 test kit (Beyotime, China), 1 mL of cell suspension was taken, 2μL JC-1 working liquid (5μM) was added, mixed evenly, and incubated under 37 °C and 5% CO2 for 20 min away from light.

Techniques: Derivative Assay, Expressing, Control, Microscopy, Staining, Fluorescence